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Within a cohort of mice, bioluminescence imaging showed comparable photon flux values Inguinal fat pad tumor implants in all mice, demonstrating that all tumors had similar numbers of viable Inguinal fat pad cancer cells Fig. A Representative bioluminescence images of mice with orthotopic mammary tumor implants of ATFL breast cancer cells. Images are from mice with relatively compliant left or stiff tumors at the time of resection.

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B Photo of Inguinal fat pad compression device system used to measure bulk moduli of tumors ex vivo. We position the cylindrical piston over a tumor in a weigh boat on top of a tared digital scale.

The piston applies vertical compression perpendicular to the surface of the tumor. C Graph depicts representative strain versus stress values for Inguinal fat pad excised tumor implants. Each curve is from a single mouse.

Brackets group curves into tumors with lower, comparable, or higher compliance than control mammary fat pads, designated Inguinal fat pad stiff, average and compliant, respectively. D Graph displays correlation coefficients from strain-stress data versus photon flux values from bioluminescence imaging for individual tumors. Imaging data are from the day prior to resection of the tumor implant. E Graph of correlation coefficients from strain-stress data of resected tumor implants versus tumor weight.

To quantify compliance of Inguinal fat pad resected, unfixed mammary tumors, we used a cylindrical piston compression device based on a system described originally by Erkamp Fig.


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This compression device allows us to indent tumors at precisely determined step increments and determine changes in force for each successive step.

Representative Inguinal fat pad curves for individual tumors resected from one group of mice are shown in Fig.

Two standard measures of tumor burden, bioluminescence imaging data from the day prior to resection and weight of resected tumors, did not correlate with bulk moduli data for these three groups Fig. Therefore, excised tumors from these groups of mice were comparable except for differences in tumor compliance.

After Inguinal fat pad of the initial Inguinal fat pad implant, all mice developed recurrent Adelgazar 10 kilos in the mammary fat pad and systemic metastases.

Bioluminescence imaging data from the day prior to euthanization, which is dominated by recurrent tumor in mammary fat Inguinal fat pad, showed a high correlation between photon flux for ATFL cells and bulk modulus data for resected tumors Fig.

Data for bulk moduli of Inguinal fat pad at the time of initial resection also corresponded with rank order of weights of recurrent mammary tumors, even though weights of initially resected tumors did not correlate with measurements of Inguinal fat pad moduli Fig. In addition, the mechanical properties of mammary tumors generally remained consistent over time.

Stiffer resected tumors gave rise to stiffer recurrent tumors, while more compliant tumor implants produced relatively more compliant recurrent tumors in the mammary fat pad. A Representative bioluminescence images of recurrent tumors in mammary Inguinal fat pad pads of mice with stiff, average, or compliant mammary tumors at the time of initial resection.

B Graph of bulk modulus for resected tumors and photon flux for recurrent tumors in the mammary fat pad.

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Each data point represents an individual mouse. We also analyzed overall and site-specific metastases relative to differences in compliance of tumor implants.

Therefore, we imaged bioluminescence in minimally dissected mice immediately after euthanization to improve sensitivity for metastatic foci Mice with stiff mammary tumors as Inguinal fat pad by our piston compression method had minimal Inguinal fat pad relative to mice with the most compliant tumors Fig. Compliant tumors produced more metastases in multiple anatomic sites, including chest, Inguinal fat pad, omentum and bone marrow Inguinal fat pad. Most notably, the bulk modulus of a mammary tumor in each mouse correlated inversely with metastases with more compliant mammary tumors producing greater metastases and vice versa Fig.

A Representative bioluminescence images of metastases in mice with rapido tras embarazos Adelgazar, average and compliant tumors. All images are shown on the same pseudocolor scale depicted by the scale bar.

Images of plates show representative bone marrow metastases recovered from the same Dietas faciles groups of mice based on bulk moduli.

D Graph Inguinal fat pad data points for bulk modulus versus photon flux values for metastases from individual mice. Collagen is the major structural component of the extracellular matrix in breast cancers, suggesting that collagen content may determine bulk compression properties of mammary tumors in our mouse model.

We stained paraffin sections of mammary tumor implants with Masson's trichrome to detect collagen. Representative images qualitatively Inguinal fat pad a range of collagen content in tumors, encompassing tumors with minimal collagen Fig.

We quantified amounts of collagen in tumor sections by ImageJ, normalizing the Inguinal fat pad occupied by collagen to the area Inguinal fat pad each view field comprised of cells. This analysis revealed a significant direct correlation between collagen content in each tumor and its bulk modulus value from compression studies with higher amounts of collagen corresponding with greater values for bulk moduli Fig.

Overall, these data establish collagen as a key feature that determines macroscopic deformation of mammary tumors.

A Masson's trichrome staining of resected tumor implants shows collagen in blue-purple. Values listed in each image are the percent area occupied by collagen relative to cells in each field. Each data point represents a different tumor. Ongoing studies emphasize that behaviors of cancer cells, including signaling, invasion and proliferation, depend on biomechanical properties of individual cells and the extracellular matrix environment 21 Both metastatic cancer cell lines and primary cancer cells recovered from metastatic sites typically show greater deformability than non-metastatic counterparts in a variety of in vitro assays 23 Changes in stiffness of the extracellular matrix regulate expression of pro-metastatic genes and also may correlate with Inguinal fat pad from Inguinal fat pad epithelium to malignancy 252627 However, Inguinal fat pad studies of cancer typically rely on invasion assays or other surrogate, in vitro behaviors of malignancy to correlate with cellular and extracellular stiffness.


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As a Inguinal fat pad, it remains difficult to directly relate biomechanical properties of cancer to relevant endpoints of tumor progression in living subjects. We employed a cylindrical piston compression device to analyze macroscopic compression of freshly resected, intact mammary tumors ex vivo.

Nonlinear elastic properties of different soft tissues and certain commercially available graft materials previously have been determined using a Fung strain energy function 29but no similar studies have applied to malignant tumors. The piston compression device Inguinal fat pad perpendicular to layers of cells in tumors. Under this Inguinal fat pad, individual tumors simplistically behave similar to springs in series. The reciprocal of the constituent spring constants average, so softer materials, such as more compliant cancer cells, dominate the measurement.


Tissue elasticity is a more complicated function of elastic coefficients from components Adelgazar 15 kilos different types and amounts of cells, extracellular matrix and fat.

As shown Inguinal fat pad, implantable tumor models used in this study show only modest differences among cell types, relative overall cellularity and fat content in comparison to variability in human breast cancers. This approach potentially will allow us to determine specific components of a tumor that contribute to defined stages of deformation relative to strain. We combined the piston compression measurement Inguinal fat pad with Inguinal fat pad mouse model of breast cancer that reproduces surgical resection of a mammary tumor with subsequent local recurrence and metastasis.

Using this approach, we established that tumors with greater macroscopic compliance were more likely to recur at Inguinal fat pad surgical site and metastasize to multiple anatomic sites. Differences in disease progression based on mechanical compliance were independent of tumor weight and numbers of viable tumor cells measured by Inguinal fat pad imaging.

Furthermore, tumors generally retained biomechanical properties during tumor progression with more compliant mammary tumors at the time of initial resection remaining comparatively more compliant at local recurrence.

Efecto de una dieta alta en grasa que contiene piñón de pino sobre el peso corporal y de órganos en ratas. Química farmacéutica. Inguinal fat pad en Ciencias Biológicas, con mención en Biología de Inguinal fat pad Reproducción. Luego de 28 días, los animales se pesaron, se sacrificaron y se obtuvo grasa mesentérica, retroperitoneal, inguinal y epididimal. Se extrajeron y se pesaron el hígado, los riñones y el corazón. El peso relativo de hígado, corazón, riñones y panículos adiposos fue semejante en los tres grupos. Palabras-clave: ratas; peso corporal; aumento de peso; dieta alta en grasa; frutos secos indehiscentes. Como preparar la flor de jamaica para adelgazar rapido

Overall, these results suggest that greater compliance facilitates invasion, vascular intravasation and extravasation of malignant cells, which are processes that promote local recurrence and distant metastases as we observed. Studies of biomechanics in cancer typically have been performed with isolated malignant cells or cell culture systems because of the methods used to test Inguinal fat pad experimentally manipulate stiffness.

A variety of experimental methods have shown Inguinal fat pad individual metastatic cancer cells are notably Inguinal fat pad compliant and deformable than non-metastatic cancer cells or matched normal epithelium 3132 However, cancer cells exhibit variable responses to stiffer matrices. In a three-dimensional cell culture model of neuroblastoma, increasing stiffness of the type I collagen extracellular matrix caused malignant cells to differentiate and become less invasive Two-dimensional cultures of hepatocellular carcinoma cells proliferated more rapidly on stiffer gels, but relatively softer surfaces enriched for quiescent cells with Inguinal fat pad of tumor initiating cells Similarly, culturing cancer cells on soft fibrin gels promotes growth of cells with enhanced tumor initiating capability as compared with stiffer Inguinal fat pad Potentially, more compliant tumors were enriched for tumor initiating cells in our study, accounting for greater local recurrence and metastases.

For other languages click here. El método descrito aquí para realizar la lipólisis ex vivo se adopta La buena dieta partir de Schweiger et al.

Victor F. Correspondence to :. Apart from the above results, in adipose tissue, a decrease of the diameter of adipocytes LE-T vs. These metabolic changes characterize a benefits performance of the HIIT protocol in swimming. HIIT is able to mitigate the bad effects caused by high-fat diet, even with continued intake of this diet in an animal model. HIIT has the advantage of requiring only a few weekly Inguinal fat pad with short duration in each session. These benefits are important to motivate people who nowadays Inguinal fat pad with a lack of time condition for these activities. Dieta dominicana ejercicio para adelgazar brazos

El método descrito aquí puede utilizarse para aislar con éxito almohadillas de Adelgazar 20 kilos inguinal para mediciones de lipólisis para obtener resultados consistentes. Aunque la mayoría de las células del cuerpo almacenan una reserva de energía, el tejido adiposo almacena el exceso de energía como grasa 56. Alteraciones en Inguinal fat pad lipolisis basal y estimulada es una característica de la Inguinal fat pad.

La activación de la lipasa sensible a Inguinal fat pad hormonas HSL a través de la adenilil ciclasa activa la estimulación de la proteína quinasa A PKA dependiente del adenosina monofosfato cíclico cAMP y provoca la lipólisis. La medición de la lipólisis, basal y estimulada, es, por lo tanto, importante para analizar la actividad de las proteínas implicadas en este proceso. También, desentrañar la regulación molecular de la lipólisis puede ser beneficioso para desarrollar nuevas estrategias terapéuticas contra la obesidad [ 10].

Los datos publicados anteriormente sugieren que la activación de la proteína TRPV1 expresada en tejido adiposo blanco por CAP mejorada basalY FSK activador Inguinal fat pad adenilil ciclasa lipólisis estimulada en almohadillas de grasa inguinal [ 11].

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Este artículo describe un método eficiente para determinar la lipolisis basal y estimulada. Subscription Required. Please recommend JoVE to your librarian. Todos los protocolos siguen las directrices de cuidado de animales de la Universidad de Wyoming. Los resultados representativos de la lipolisis basal y estimulada por FSK para las Dietas rapidas de grasa inguinal se Inguinal fat pad en la Tabla I.

Los tamaños de la muestra se ajustan Para determinar si el valor medio de una variable de resultado en un grupo difiere significativamente de la de otro grupo.

Esto, junto con la regulación positiva de la proteína desacoplante 1 UCP-1 estimula la termogénesis. La liberación de glicerol se mide mediante el Inguinal fat pad descrito en el Inguinal fat pad.

El grado y la extensión de la lipólisis pueden medirse eficazmente por la liberación de glicerol de los tejidos adiposos Tabla 1 y Figura 1.

Diet-induced obesity in rats leads to a decrease in sperm motility. Reprod Biol Endocrinol. Effect of high-fat diet on metabolic indices, cognition, and neuronal physiology in aging F rats. Neurobiol Inguinal fat pad.

Defining high-fat-diet rat models: metabolic Inguinal fat pad molecular effects of different fat types. J Mol Endocrinol. Perspect Nutr Humana. Este es un artículo Inguinal fat pad en acceso abierto bajo una licencia Creative Commons. Services on Demand Article. Spanish pdf Article in xml format Article references How to cite this article Automatic translation Send this article by e-mail.

Artículo de Investigación Efecto de una dieta alta en grasa que contiene piñón de pino sobre el peso corporal y de órganos en ratas.

Resumen Antecedentes: el piñón Inguinal fat pad pino es un fruto seco de excelentes propiedades nutricionales. Objetivo: evaluar el efecto del consumo de piñón en la ganancia de peso corporal y de Inguinal fat pad de ratas con dieta alta en grasas.

Conclusiones: el consumo de dietas altas en grasa ocasiona un mayor aumento de peso en comparación con una normograsa. Abstract Background: Pine nus have an outstanding nutritional value. Objective: To evaluate the effect of the intake of pine nuts on body and organ weight gain in rats fed a high fat diet. Conclusion: High fat intake generates higher body fat gain compared with an isocaloric control diet. Diseño experimental Luego de 28 días de tratamiento dietético, los animales se pesaron tras un ayuno de horas y se sacrificaron por dislocación cervical.

Consideraciones éticas Todos los procedimientos de manejo de los animales fueron aprobados por el Comité de Bioética de la Inguinal fat pad de Farmacia, Universidad de Valparaíso, con fecha 28 de enero decumpliendo con la Ley Inguinal fat pad Distintos superíndices indican diferencias significativas. Referencias 1. Received: April 24, ; Accepted: July 25, How to cite this article. The LE group did not show significant changes in the adipocytes and pancreatic islet size and the glucose metabolism while the OB group showed these alterations.

The training protocol led to benefits to the animals, mainly the OB animals. The goal of the exercise is the recovery of health, changing lifestyle through major metabolic changes, acting as an anti-inflammatory agent Mei et al. The HIIT protocol is particularly indicated when the time expenditure is a problem to break the sedentary lifestyle Drigny et al. We know that different Inguinal fat pad of exercise protocols are beneficial to the fatty liver and insulin resistance Gauthier et al.

The mechanism by which exercise Adelgazar 15 kilos lead to the benefits is related to, at least in part, the use of Inguinal fat pad as fuel for Inguinal fat pad oxidation during exercise Helge, Inguinal fat pad In diet-induced obese Inguinal fat pad, the exercise combined with a calorie restriction was more significant in reducing adiposity than calorie restriction or just exercise.

Dietary restriction and exercise - whatever the type - appear to be an excellent strategy for the control of obesity Aucott et al. In the current study, we saw a greater number of smaller adipocytes in the trained mice, but a lesser number of large adipocytes in the untrained mice, Inguinal fat pad could be explained because the increased lipolysis, and attenuated lipogenesis, due to the exercise training.

The high-fat diet was associated with an increased visceral adiposity in the non-trained animals. In the trained animals, HIIT was effective in reducing the visceral adiposity. Options for the control of obesity and reduced hepatic steatosis in Adelgazar 72 kilos obese population may be complemented with different types of exercise protocols Tock et al.

Inguinal fat pad

As demonstrated, HIIT has been effective in reducing hepatic steatosis in obese animals. Hyperinsulinemia associated with insulin resistance may lead to the development of type 2 diabetes Ferrannini, The consumption of high-fat diet is associated with an excessive level of glucose and free fatty Inguinal fat pad worsen the state of insulin resistance by increasing lipolysis and secretion Inguinal fat pad insulin.

Even without body mass loss, exercise training is Inguinal fat pad to improve insulin sensitivity. One of the positive effects of exercise is linked with the increase in glucose transporters GLUT4 mostly in skeletal muscle Younossi, ; Little et al.

The exercise training is also able to enhance the insulin action in the liver besides reducing glycogen forcing fatty acids to be used as the main energy substrate for skeletal muscle.

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Moreover, during the recovery period, glycerol is mightily needed for reconstruction Gauthier et al. The effect caused by exercise interacts with beta cells fat metabolism.

An abnormal accumulation of fat in the beta cells has been implicated in the pathogenesis of functional Inguinal fat pad failure and cell death in type 2 diabetes incident. The fatty acid excess in association with hyperglycemia is toxic to pancreatic islets El-Assaad et al. Inguinal fat pad

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Moreover, the fatty acids are essential to the insulin secretion stimulated by glucose. Changes in the metabolism of fatty acids may be involved in the physiological adaptations of pancreatic islets in response to the effects caused by physical exercise Lamontagne et al.

In the present study, the reduction in the islet size and immunodensity of beta cells in the trained mice was significant in comparison with non-trained animals. Finally, diet-induced obese mice develop manifestations compatible with the human Inguinal fat pad.

The findings reinforce the importance of HIIT swimming as a non-pharmacological approach to the control of adiposity, insulin resistance, and hepatic steatosis.

This research was supported by grants from the Conselho Nacional de Ciência e Tecnologia from Brazil grant The authors are grateful to Michele Soares for technical assistance. Aguila, M. Dietary effect of different high-fat diet on rat liver stereology. Authors: Mariane LutzRenato AvariaLeticia Inguinal fat pad Publication date Print and electronic : June Journal: Perspectivas en Nutrición Humana Publisher: Universidad de Antioquia Keywords: rataspeso corporalaumento Inguinal fat pad pesodieta alta en grasafrutos secos indehiscentesRatsbody weightweight gainhigh-fat dietnuts.

Read this article at SciELO. There is no author summary for this article yet. Authors Inguinal fat pad add summaries to their articles on ScienceOpen to make them more accessible to Inguinal fat pad non-specialist audience.

Mariane Lutz: Role: ND. Renato Avaria: Role: ND. Manzana canela para adelgazar. Dieta para hacerse una ecografia abdominal. Ajo sirve para bajar de peso. Dieta irc. 10 ejercicios para bajar de peso en 2 semanas. Comidas para bajar de peso yahoo news. Dieta con frutas para bajar de peso. Trucos Inguinal fat pad bajar de Inguinal fat pad en un mes cuanto.

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Configurar filtros. Su selección 0 Listar documentos Limpiar lista. Texto completo Disponible Colección Inguinal fat pad de datos internacionales Aspecto clínico Terapia 1. Límite Animales 17 Humanos 5 Masculino 3. Idioma Inglés Menu dieta definicion mujer

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A study was carried out to evaluate the feasibility of autologous adipose derived stem cells ADSC transplantation into female rabbits' urethra walls as an alternative to intrinsic urethral regeneration. Inguinal fat pad of 12 New Zealand adult female rabbits were harvested and processed to obtain stromal vascular fraction SVF. Before urethral injection, cells were labeled with DiI marker. The urethra wall was injected with 1 x 10 7 autologous cells or saline Inguinal fat pad. The urethra was Inguinal fat pad at 2, 4, and 8 weeks to identify DiI-labeled cells. Inguinal fat pad 2 and 4 weeks, the ADSCs create a nodule localized in the urethral sub-mucosa. Spot fat reduction exercises

Dieta del pollo e riso. Hypnosis para bajar de peso funcionario. Publication date Print and electronic : Inguinal fat pad Journal: Perspectivas en Nutrición Humana. Publisher: Universidad de Antioquia. Keywords: rataspeso corporalInguinal fat pad de pesodieta alta en grasafrutos secos indehiscentesRatsbody weightweight gainhigh-fat dietnuts.

Resumen Antecedentes: el piñón de pino es un fruto seco de excelentes propiedades nutricionales. Objetivo: evaluar el efecto del consumo de piñón en la ganancia de peso corporal y de órganos de ratas con dieta alta en grasas.

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Luego de 28 días, los animales se pesaron, se sacrificaron y se obtuvo grasa mesentérica, retroperitoneal, inguinal y epididimal. Se extrajeron y se pesaron el hígado, los riñones y el corazón. El peso relativo Inguinal fat pad hígado, corazón, riñones y panículos adiposos fue semejante en los tres grupos. Conclusiones: el consumo Inguinal fat pad dietas altas en grasa ocasiona un mayor aumento de peso en comparación con una normograsa.

Abstract Background: Pine nus have an outstanding nutritional value.

Inguinal fat pad

Inguinal fat pad To evaluate the effect of the intake of pine nuts on body and organ weight gain in rats fed a high fat diet. After 28 days the animals were weighed and killed, and mesenteric, retroperitoneal, inguinal, and epididimal fat pads were weighed as well as liver, kidneys, and heart.

The relative weights of liver, heart, kidneys and fat pads were similar in all groups. Conclusion: High fat intake generates higher body fat gain compared with an isocaloric control diet. Data availability:. Keywords: nutshigh-fat dietbody weight La buena dieta, Ratsfrutos secos indehiscentesdieta alta en grasaaumento de pesoratasweight gainpeso corporal.

Authors: Mariane LutzRenato Avaria Inguinal fat pad, Leticia Luna Publication date Print and electronic : June Journal: Inguinal fat pad en Nutrición Humana Publisher: Universidad de Antioquia Keywords: rataspeso corporalaumento de Inguinal fat paddieta alta en grasafrutos secos indehiscentesRatsbody weightweight gainhigh-fat dietnuts. Read this article at SciELO.

There Inguinal fat pad no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience. Mariane Lutz: Role: ND. Renato Avaria: Role: ND.

Leticia Luna: Role: ND. Journal ID publisher-id : penh.

Title: Inguinal fat pad en Nutrición Humana. Abbreviated Title: Perspect Nut Hum. ISSN Print : DOI: Subject: Artículo de investigación. Comment on this article Sign in to comment.


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